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This volume includes comprehensive and up-to-date coverage of all nucleic acid-specific steps used in X-ray crystallography, from macromolecule production to structure determination. Chapters dedicated to RNA preparation and crystallogenesis will be of interest to beginners, while chapters focused on data collection, phasing and refinement will be particularly useful to researchers with a higher level of expertise. Several functional case studies are also presented in the last part of the book. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and thorough, Nucleic Acid Crystallography: Methods and Protocols presents protocols that are aimed at both researchers and students who are interested in the structural biology of DNA or RNA, alone or in complex with proteins or ligands.  .

Nucleic acids. --- Nucleic Acid Chemistry. --- Polynucleotides --- Biomolecules

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Molecular biology. --- Molecular biology --- Experiments. --- Experiments --- History. --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology

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This book presents an overview of the most relevant protein knockdown techniques. Readers will find a description of the generation and use of recombinant human antibodies, ER intrabodies and peptides as well as a description of the working mechanism and potential of the inhibitory action of each of these molecules. The book will also describe the selection and activity of a number of phosphatases, aptamers and allosteric modulators, focusing on A G protein coupled receptors. The book starts with a chapter on the in silico prediction of target-inhibitor interactions. Key aspects of this book are: - Selection strategies for monoclonal and recombinant human antibodies - Selection strategies for ER intrabodies, peptides and aptamers - Examples of the most efficient inhibitors and their applications in protein biochemistry and cell biology - Antibodies for cancer therapy and inhibitors of angiogenesis. The book will be of great interest to scientists and students working in the life sciences on all areas related to protein biology. The variety of methods to modulate the activities of specific proteins which are introduced here will be of great benefit to the reader who is interested in general protein research or to readers who have very specific protein related questions.

Medicine. --- Immunology. --- Biomedicine. --- Proteins --- Synthesis --- Inhibitors. --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Immunobiology --- Life sciences --- Serology

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This book will take an evidence-based approach to current knowledge about biomolecules and their place in our lives, inviting readers to explore how we know what we know, and how current gaps in knowledge may influence the way we approach the information. Biomolecular science is increasingly important in our everyday life, influencing the choices we make about our diet, our health, and our wellness. Often, however, information about biomolecular science is presented as a list of immutable facts, discouraging critical thought. The book will introduce the basic tools of structural biology, supply real-life examples, and encourage critical thought about aspects of biology that are still not fully understood.

Life sciences. --- Science. --- Systems biology. --- Cell biology. --- Life Sciences. --- Cell Biology. --- Systems Biology. --- Science, general. --- Molecular biology. --- Biomolecules. --- Biological molecules --- Molecular biochemistry --- Molecular biophysics --- Molecules --- Molecular biology --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology --- Cytology. --- Biological models. --- Science, Humanities and Social Sciences, multidisciplinary. --- Models, Biological --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Computational biology --- Bioinformatics --- Biological systems

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This book spans diverse aspects of modified nucleic acids, from chemical synthesis and spectroscopy to in vivo applications, and highlights studies on chemical modifications of the backbone and nucleobases. Topics discussed include fluorescent pyrimidine and purine analogs, enzymatic approaches to the preparation of modified nucleic acids, emission and electron paramagnetic resonance (EPR) spectroscopy for studying nucleic acid structure and dynamics, non-covalent binding of low- and high-MW ligands to nucleic acids and the design of unnatural base pairs. This unique book addresses new developments and is designed for graduate level and professional research purposes.

Biochemistry --- Chemistry --- Physical Sciences & Mathematics --- Nucleic acids. --- Polynucleotides --- Biomolecules --- Cytology. --- Biochemical engineering. --- Nucleic Acid Chemistry. --- Cell Biology. --- Biochemical Engineering. --- Bio-process engineering --- Bioprocess engineering --- Biotechnology --- Chemical engineering --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Cell biology.